The purpose of this best practice guideline (BPG) is to provide nurses and other members of the interprofessional team with evidence-based recommendations on foundational, inclusive care practices for 2SLGBTQI+ people. This BPG is also to be used to enhance the safety of health-service organizations and academic organizations for 2SLGBTQI+ people through the adoption of evidence-based practices. Safe and inclusive environments enable nurses and other members of the interprofessional team to optimize health outcomes for those receiving care. The BPG addresses foundational inclusive care practices, chronic disease prevention and management, health promotion and health care for 2SLGBTQI+ people.